Dr. Eva Doka#
AFFILIATION: National Institute of Oncology, Budapest, Hungary
LINK TO WEBPAGE: http://www.onkol.hu/en/department-molecular-immunology-and-toxicology-mito
FIELD OF SCHOLARSHIP: Chemistry
BIOGRAPHICAL NOTE:
Place of studies: University of Debrecen
MSc in Chemistry, 2012
BSc in Mathematics, 2013
PhD in Chemical Sciences, 2016
Thesis: Experimental and Modelling Studies on the Reactions of the Sulfate Ion Radical
Supervisor: Dr. Gábor Lente, Dept. of Inorganic and Analytical Chemistry
Current occupation:
Chemist
National Institute of Oncology, Dept. of Molecular Immunology and Toxicology, Budapest, Hungary
Foreign studies:
Karolinska Institutet, Stockholm, 1 month
Tohoku University, Sendai, Japan, 2 months
DETAILS OF RESEARCH:
I’ve been trained in the experimental and computational methods of solution phase reaction kinetics, and the studies of the mechanism of complex redox reactions, mainly regarding environmental chemistry.
I’m currently involved in fundamental biochemical research. Our group has two main lines of interest, the biological actions of hydrogen sulfide, a newly recognized endogenously produced signalling molecule, and the redox regulation of thiol proteins by various posttranslational modifications. A close linker between these subjects are the formation of protein persulfide and polysulfide moieties on cysteine side chains, which was previously shown to regulate enzymatic activities. In our laboratory, we developped a novel experimental method for the detection of protein persulfidation from biological materials such as living cells or tissue samples. Furthermore, we showed that global persulfidation level is tightly regulated by the major disulfide reductase machineries of the cells, the thioredoxin and the glutathione systems. We are currently examining the consequences of this newly recognized functions of the NADPH dependent reductase system on cellular signaling and redox regulation.
THREE KEY PUBLICATION REFERENCES:
Doka, E., et al. Science Advances, 2016, 2, e1500968.
Doka, E., Arnér, E. S. J., Schmidt, E. E., Nagy, P. Methods in Molecular Biology, in press
Peralta, D., et al. Nature Chemical Biology, 2015, 11, 156-63.
